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. 2021 Jul 29;9(8):833. doi: 10.3390/vaccines9080833

Figure 2.

Figure 2

Production of recombinant scFv. (A) The expression of recombinant scFv in Expi293F™ cells was confirmed by Western blotting. The expected size of the recombinant scFv is approximately 30 kDa. Lane 1: The His-tagged PEDV S protein was used as a positive control. Lane 2: The cell lysates from non-transfected HEK 293 cells. Lane 3: The cell lysates from scFv-transfected cells. Lane 4: The supernatant from non-transfected cells. Lane 5: The supernatant from scFv-transfected Expi293F™ cells. L: Protein ladder. (B) Size-exclusion chromatogram of the recombinant scFv purified by IMAC. UV280 detected the protein content in mAU. The black arrowhead indicates the expected major peak of the scFv. (C) The expression and purity of the purified scFv in Expi293F™ cells. Lane 1: two-fold dilution of the purified scFv. Lane 2: 10-fold dilution of the purified scFv. Lanes 3–8: two-fold serial dilutions of standard BSA from 2 mg/mL to 0.0625 mg/mL.