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. 2021 Jul 29;9(8):833. doi: 10.3390/vaccines9080833

Figure 3.

Figure 3

ICC and IFA of scFv against PEDV-infected cells. The purified scFv was used as the primary antibody to stain PEDV-PT (G2 PEDV) and CV777(G1 PEDV). The DAB coloration substrate was added for visualization in ICC (A–D); DAPI was used to stain the nuclei in IFA (E–H). (A,E) The immunostainings of scFv against PEDV-PT-infected cells. (B,F). The immunostainings of scFv against CV777-infected cells. (C,G). The controls of immunostainings of PEDV-PT-infected cells. (D,H) The controls of immunostainings of CV777-infected cells. The bars represented 100 μm in length.