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. 2021 Jul 29;9(8):833. doi: 10.3390/vaccines9080833

Table 2.

The primers used in the PCR reactions. Along with the common forward primer (ISPCR), the primers for variable kappa (mIgKpcr), variable lambda (mIgLpcr) light chains, and variable heavy chain (mIgHGpcr) were utilized to amplify specific sequences.

Primer Name Primer Sequence
ISPCR 5′-AAGCAGTGGTATCAACGCAGAG-3′
mIgKpcr 5′-ACATTGATGTCTTTGGGGTAGAAG-3′
mIgLpcr 5′-ATCGTACACACCAGTGTGGC-3′
mIgHGpcr 5′-GGGATCCAGAGTTCCAGGTC-3′