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Flow-chart of the protocol. Schematic flow-chart of the protocol, divided into 3 distinct parts. Part 1: Cultivation of producer cells and collection of 550 mL HBV particle-rich supernatant per HYPERflask. Part 2: Purification and concentration of HBV particles via heparin-affinity chromatography in a 17 mL eluate. Part 3: Buffer exchange, concentration and purification of HBV particles via sucrose gradient ultracentrifugation resulting in a 2 mL high-titer HBV fraction.
