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. 2021 Jul 30;13(8):1503. doi: 10.3390/v13081503

Figure 6.

Figure 6

Toxicity and stability of high-titer HBV stocks. HBV was purified with the protocol described herein and aliquoted. HepG2-NTCP were then infected at an MOI of 100 and infection was analyzed by HBeAg ELISA of supernatant 9 days post-infection [4]: (a) To examine the inhibiting and toxic effect of sucrose on in vitro infection, sucrose was added to HBV aliquots at different concentrations prior to HepG2-NTCP infection. # = sucrose concentration toxic to cells; (b) HBV aliquots were exposed to multiple freeze-thaw cycles prior to HepG2-NTCP infection; (c) HBV aliquots were exposed to different storage conditions for 24 h prior to HepG2-NTCP infection. Statistical significance was determined using Student’s t test, ** p < 0.01, * p < 0.05, n.s. not significant.