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. 2021 Aug 19;13(8):1647. doi: 10.3390/v13081647

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Second Chimera Removal Method in SAM Refiner, Covariant Deconvolution. (A) Calculations of the singles/expected abundance and abundance ratio for one of the unique sequences from Sup. 5 and the abundances from Sup. 7. Lines connect the singles and their abundance to the same in (B). (B) Calculations for determining if a unique sequence passes the initial check. Sequences pass when they have an abundance/singles ratio of 1 or greater. (C) Passed sequences are processed in order of greatest ratio to least. Counts of the sequence are set to the counts of the least abundant single variant, and that count is then removed from all single variants in that sequence.