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. 2021 Aug 19;13(8):1647. doi: 10.3390/v13081647

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Relative Abundance of Reference and Variant SARS-CoV-2 Sequences Observed in Fenton, MO sewershed from February to March. Results from sequencing of spike amplicons of the NTD, RBD and S1S2 junction regions are shown. Lines of short dashes connect values obtained by the chimera removed method, lines of long dashes connect values obtained by the covariant deconvolution method. All amplicons show a population shift from the reference with D614G to B.1.1.7 sequences with the appearance of P.1 sequences at the last time point. Additionally, known common polymorphisms T478K and L452R were observed from the RBD amplicons. RT-PCR for the RBD amplicon was performed in duplicate for some samples.