Table 2.
Antigenic analysis of different domains of CSFV E2 protein by IFA with E2-specific mAbs a.
| Domain | Strain b | mAbs against E2 | ||||||
|---|---|---|---|---|---|---|---|---|
| Domain B/C | Domain A | |||||||
| CSFV c | BDV | CSFV | ||||||
| 1C7A1 | 3C1E12 | WH220 | WH304 | WS381 | WS384 | WH303 | ||
| B/C (aa 690–779) |
TD/96 | + | + | + | + | + | + | − |
| 94.4 | − | + | − | + | − | + | − | |
| LPC | − | − | + | + | − | − | − | |
| B/C/D (aa 690–806) |
TD/96 | + | + | + | + | + | + | − |
| 94.4 | − | + | − | + | − | + | − | |
| LPC | − | − | + | + | − | − | − | |
| C/D/A (aa 717–869) |
TD/96 | − | − | − | − | − | − | + |
| 94.4 | − | − | − | − | − | − | + | |
| LPC | − | − | − | − | − | − | + | |
| D/A (aa 770–869) |
TD/96 | − | − | − | − | − | − | + |
| 94.4 | − | − | − | − | − | − | + | |
| LPC | − | − | − | − | − | − | + | |
| B/C/D/A (aa 690–869) |
TD/96 | + | + | + | + | + | + | + |
| 94.4 | − | + | − | + | − | + | + | |
| LPC | − | − | + | + | − | − | + | |
| Full-length (aa 690–1062) |
TD/96 | + | + | + | + | + | + | + |
| 94.4 | − | + | − | + | − | + | + | |
| LPC | − | − | + | + | − | − | + | |
a Results were interpreted as positive (+) (grey highlights) or negative (−). b TD/96, 94.4 and LPC indicated CSFV strains TD/96/TWN (genotype 2.1), 94.4/IL/94/TWN (genotype 3.4) and LPC/AHRI (genotype 1.1), respectively. c Derived from CSFV or BDV.