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. 2021 Aug 7;13(8):1562. doi: 10.3390/v13081562

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Genotyping and origin of the PEDV strains 17GXCZ-1ORF3d and 17GXCZ-1ORF3c based on different genes. Phylogenetic trees were constructed based on the aligned full-length genomes (a) S protein and (b) ORF3 protein (c) by using the neighbor-joining method from MEGA 5.2, with 1000 bootstrap replicates. Scale bars represent the branch lengths measured by the number of substitutions per site. Each PEDV strain is indicated in the following format: country of origin (three letter code: CHN, China; JPN, Japan; KOR, Korea; MEX, Mexico; SUI, Switzerland; and USA, the United States)/strain name/year of sample collection (Genbank accession number). Group 1, G2a subgroup, G2b subgroup and Group 3 are coded in red, green, pink and yellow, respectively. The triangle symbols represent the strains obtained in this study.