TABLE 3.
Tf1 cDNA recombination and transposition frequencies of wild type and pst1-1 mutant strain
| Strain | Description | % Transposition (5-FOAr-G418r colonies/5-FOAr colonies) | % cDNA recombination (G418r colonies/YES colonies) |
|---|---|---|---|
| YHL1282 | Wild-type strain containing Tf1-neoAI | 2.1 (276/13,368) | 0.43 (1,734/398,200) |
| YHL1554 | Wild-type strain containing Tf1-neoAI with IN frameshift | 0.1 (10/11,640) | 0.13 (466/351,639) |
| YHL1836 | Wild-type strain containing Tf1-neoAI with PR frameshift | <0.01 (0/11,412) | <0.001 (0/347,096) |
| YHL5406 | pst1-1 mutant strain containing Tf1-neoAI | 0.2 (26/12,166) | 0.05 (186/402,450) |