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. 2021 Aug 19;13(8):1650. doi: 10.3390/v13081650

Figure 1.

Figure 1

EBOV VP24 undergoes nucleocytoplasmic trafficking. (a) COS7 cells co-transfected to express the indicated proteins were fixed 24 h post-transfection before immunofluorescent staining for FLAG (red), and CLSM analysis. Representative images are shown. (b) Images such as those shown in (a) were analyzed to calculate the Fn/c for GFP (mean ± SEM, n ≥ 52 cells for each condition; data from a single assay representative of three independent assays). (c) Schematic of VP24 and truncated proteins generated. Potential NESs are indicated in yellow (sequences shown above). Location of residues that interact with Kα5 in the VP24:Kα5 crystal structure (CL1-3) [9] are shown in red. Numbering indicates positions in full-length VP24. (d,e) COS7 cells transfected to express the indicated proteins were treated 24 h post-transfection with or without LMB before live-cell CLSM (d) to determine the Fn/c for GFP (e); mean ± SEM; n ≥ 31 cells for each condition; data from a single assay representative of three independent assays. ****, p < 0.0001; No add., no addition. Scale bars, 30 μm.