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. 2021 Aug 19;13(8):1650. doi: 10.3390/v13081650

Figure 3.

Figure 3

EBOV VP24 NES is dispensable for IFN/STAT1 antagonism. (a,b) COS7 cells transfected to express the indicated proteins were treated 24 h post-transfection with or without IFN-α (30 min) before fixation, immunofluorescent staining for STAT1 (red) and CLSM (a) to determine the Fn/c for GFP and STAT1 (b); mean ± SEM, n ≥ 36 cells. Arrowheads indicate cells with detectable GFP expression. Scale bars, 30 μm. (c) HEK293T cells co-transfected with pISRE-Luc and pRL-TK, and plasmids to express the indicated GFP-fused proteins, were treated 8 h post-transfection with or without IFN-α (16 h) before determination of luciferase activity (mean ± SEM; n = 4 independent assays); lower panel: cell lysates used in a representative assay were analyzed by immunoblotting (IB) for GFP. *, p < 0.05; ****, p < 0.0001; NS, not significant; No add., no addition.