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. 2021 Jul 27;13(8):1471. doi: 10.3390/v13081471

Figure 1.

Figure 1

Comparative titration of lentiviral LeGO-mOrange2 vectors produced with standard vs. codon-optimized (co) GALV-env. (a) Much lower amounts of coGALV-env as compared to GALV-env were required to obtain maximal titers. Supernatants were harvested 12, 24, and 36 h after transfection of 293T cells. As evident, maximal titers were in all cases obtained with >20 times lower amounts of coGALV-env as compared to GALV-env; (b–h) titration experiments for seven additional, independent vector productions (only the 24 h data point is shown). Means and standard deviations of triplicates are shown. Small error bars are part hidden behind data point symbols.