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. 2021 Jul 31;13(8):1517. doi: 10.3390/v13081517

Figure 1.

Figure 1

An in vitro binding assay to measure alphavirus Cp binding to fluorescently labeled RNA. (a) Fluorescence signal across serial dilutions of Alexa488-labeled RNA. Inset: zoom in on lower RNA quantities. Red dot represents the RNA quantity used in subsequent binding experiments. Individual data points from n = 2 experiments are shown. Line represents their mean. (b) 1 µg of purified SFV full length Cp or the Cp C-terminal domain (CTD) was subjected to SDS-PAGE and visualized by Coomassie staining. Image is representative of n = 5 SFV full length Cp and n = 1 SFV Cp CTD purifications. (c) Binding of increasing amounts of full length SFV Cp or SFV Cp CTD to 10 nM Alexa488-RNA. Data points represent the average from n = 2 independent experiments and bars represent the range. Data points are fit with nonlinear regression specific binding with Hill slope in Prism.