Figure 4.

Specific recognition of site #1’s stem loop is conserved across other SFV complex alphavirus Cps. (a) 1 µg of SFV, RRV, and CHIKV purified Cps were subjected to SDS-PAGE and visualized by Coomassie staining. Image is representative of n = 5 SFV, n = 1 RRV, and n = 3 CHIKV Cp purifications. (b) RRV Cp binding to Alexa488-labeled site #1, Mutant, StmLp, and StmLpMutant RNAs in the presence of polyIC. Data points represent the average from n = 3 independent experiments. Error bars represent the standard deviation. Data points are fit with nonlinear regression specific binding with Hill slope in Prism. (c) As in (b), but for CHIKV Cp.