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. 2021 Jul 23;13(8):1432. doi: 10.3390/v13081432

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of APH-2 on HIV-1 Tat-mediated transcription: 293T cells were transfected with pBlue3′LTR (1 µg) only and co-transfected with pCDNA3.1 Tat 101-flag (3 µg), HA-APH-2 (3 µg), as well as mutant versions of HA-APH-2 (3 µg), (+ and − sign used to represent presence and absence of plasmid used for transfection) as shown in the figure. The graph represents the luciferase activity measured 24 h post-transfection (n = 3 ± SD). p-values were determined using a Student’s t test. * p < 0.01. Dashed lines indicate no significant difference. The bottom panel represents the cellular expression of pCDNA3.1 Tat 101-flag, HA-APH2 and HA-APH-2 mutants. Cellular beta actin expression represents the loading control.