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. 2021 Aug 3;13(8):1533. doi: 10.3390/v13081533

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Efficacy of inhibitors against VEEV TC-83 in U-87MG astrocytes. (A) U-87MG cells were treated with small molecule inhibitors at varying concentrations (μM). Cell viability was measured at 24 hpt and calculated versus the DMSO vehicle control, as described in Materials and Methods. The dotted line represents the 90% cut-off point. (B) Schematic of experimental setup. (C) U-87MG cells were treated with a single, nontoxic concentration of inhibitor or 0.1% DMSO for 2 h. Cells were infected with TC-83 at MOI of 0.1 in triplicate for 1 h. Conditioned media containing inhibitor was replaced after removal of the virus. At 24 hpi, viral supernatants were collected and evaluated by plaque assay, as described in Materials and Methods. Graph represents data from two independent experiments performed in triplicate (n = 6). * p < 0.0332, **** p < 0.0001, ns, not significant.

Efficacy of inhibitors against VEEV TC-83 in U-87MG astrocytes. (A) U-87MG cells were treated with small molecule inhibitors at varying concentrations (μM). Cell viability was measured at 24 hpt and calculated versus the DMSO vehicle control, as described in Materials and Methods. The dotted line represents the 90% cut-off point. (B) Schematic of experimental setup. (C) U-87MG cells were treated with a single, nontoxic concentration of inhibitor or 0.1% DMSO for 2 h. Cells were infected with TC-83 at MOI of 0.1 in triplicate for 1 h. Conditioned media containing inhibitor was replaced after removal of the virus. At 24 hpi, viral supernatants were collected and evaluated by plaque assay, as described in Materials and Methods. Graph represents data from two independent experiments performed in triplicate (n = 6). * p < 0.0332, **** p < 0.0001, ns, not significant.