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. 2021 Jul 23;13(8):1433. doi: 10.3390/v13081433

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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3-indoleacetonitrile inhibits influenza virus replication with a broad spectrum. (A) The protein level of H5N6 NP and PB2 was measured in A549 cells treated with an increased dose of 3-indoleacetonitrile. (B,C) The effect of 3-indoleacetonitrile on H5N6 vRNA (B) and mRNA (C) abundance at 3, 6, and 9 hpi. (D–F) Effect of 3-indoleacetonitrile on viral PB2 and NP protein level in A549 cells infected with PR8 (D), H3N2 (E), or Cal09 (F) viruses. ** p < 0.01; *** p < 0.001; calculated from three independent experiments by two-tailed Student’s t-test.