Figure 6.

Pf4r restores LasB activity and slightly increases mutant frequencies. (a,b) Pyoverdine and pyocyanin production were semi-quantified by measuring emission fluorescence at 460 nm and by absorbance at OD₆₉₅, respectively. (c) Extracellular protease activity in cell-free supernatant was measured using elastin-congo red for LasB (increase in absorbance at OD₄₉₅). Significant difference was determined using Dunnett’s multiple comparisons test. (d) The number of viable cells after 25 mM H₂O₂ treatment for 30 min and (e) percentage of the mutant frequencies of ΔPf4 and PAO1 after treatment with H₂O₂. For all assays, N = 3.