Table 1.
Information on immunohistochemical reagents and procedures.
| Marker | Clone | Supplier | Dilution of Primary Antibody/Incubation Time | Antigen Retrieval | Positive CTR |
|---|---|---|---|---|---|
| CD3 | CD3-12 | Leucocyte’s antigen laboratory, UC Davis | 1:20/overnight | Citrate buffer pH 6.00 in microwave at 750 W for 10′ | Canine lymph node—paracortical areas |
| CD79α | HM57 | Santa Cruz | 1:200/overnight | EDTA buffer pH 8.00 in microwave at 750 W for 10′ | Canine lymph node—germinal follicles |
| Iba1 | Poly-clonal | Novus Bio | 1:2000/overnight | Citrate buffer pH 6.00 in microwave at 750 W for 10′ | Canine lymph node—macrophages in medullary sinuses |
| Pan-cytokeratin | AE1/AE3 | Dako | 1:300/overnight | Citrate buffer pH 6.00 in microwave at 750 W for 10′ | Canine skin—epidermis |