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. 2021 Aug 19;13(8):1651. doi: 10.3390/v13081651

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The effect of different I-ELISA cut-off values on the categorization of sera from RVF-non-endemic countries tested negative in the virus neutralization test. Distribution of IgG I-ELISA PP values (grey area) in (A) sheep (n = 1493), (B) goats (n = 560), and (C) cattle (n = 955). Sera ordered according to ELISA PP values. Vertical lines indicate the ELISA cut-off values determined by the TG-ROC analysis (solid line) in ruminant serum panels from RVF-endemic countries and as mean plus three (dotted line) and as mean plus two (slashed line) standard deviations observed in the VNT-negative serum panels from RVF-free countries.

The effect of different I-ELISA cut-off values on the categorization of sera from RVF-non-endemic countries tested negative in the virus neutralization test. Distribution of IgG I-ELISA PP values (grey area) in (A) sheep (n = 1493), (B) goats (n = 560), and (C) cattle (n = 955). Sera ordered according to ELISA PP values. Vertical lines indicate the ELISA cut-off values determined by the TG-ROC analysis (solid line) in ruminant serum panels from RVF-endemic countries and as mean plus three (dotted line) and as mean plus two (slashed line) standard deviations observed in the VNT-negative serum panels from RVF-free countries.