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. 2021 Aug 11;13(8):1593. doi: 10.3390/v13081593

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Cluster of SARS-CoV-2 derived small RNAs. (A) Schematic representation of SARS-CoV-2 genome. The red vertical arrow indicates the identified cluster 1. The cluster 1 is located at position 1058 nt from the genome start in sense orientation at the 3′ end of the sequence coding for the non-structural protein 2 (nsp2, red bar) within open reading frame 1 (ORF1, green bar). nsp: non-structural protein; ORF: open reading frame; Structural proteins: S: spike; E: envelope; M: membrane; N: nucleocapsid. (B) Bar diagram indicating the normalized number of reads from the samples in reads per million (RPM) mapping at the cluster region (sequence: CCTTCAATGGGGGATGTCC). Control samples (negative for presence of SARS-CoV-2 by PCR-test, blue bars; mild COVID-19, light grey; severe COVID-19 (ICU), dark grey.