Table 3.

Host ribodepletion reaction mix.

Component	Amount or Volume
Total RNA (DNA removed)	1 µg
5X First-Strand Buffer *	8 µL
QIAseq FastSelect—rRNA Plant	1 µL
Nuclease-Free Water	to 40 µL

* The 5X First-Strand Buffer comes with the Invitrogen SuperScript III RT enzyme for PCR. Alternative buffers with a similar composition may be used.