Figure 3.

PNA treatment reduces PGR expression in non-KNDy ARC glutamatergic and GABAergic cells following PNA treatment. A, C, Confocal images of representative cells in the ARC (identified by DAPI, white) containing RNA transcripts for VGLUT2 (Ai) or vGAT (Ci) and PGR (Aii, Cii). Arrows depict examples of VGLUT2-positive cells co-expressing PGR transcripts. B, Mean number ± SEM of VGLUT2 (i) and PGR (ii) transcripts in glutamatergic cells. D, Mean number ± SEM of VGAT (i) and PGR (ii) transcripts in GABAergic cells. *P < 0.05. Scale bars = 10 μm.