Figure 4.

Glutamatergic and GABAergic synaptic input to KNDy neurons is reduced in PNA mice. A, C, Representative confocal images of sections triple labeled for YFP in ARC kisspeptin (Kiss) cells, (i), synaptophysin (SYN, ii) and either vGluT2 (glutamatergic inputs, Aiii) or vGaT (GABAergic inputs, Ciii) in the middle ARC of PNV male, PNV female, and PNA female mice. Arrowheads indicate synaptic terminals containing glutamate (VGLUT2 + SYN, A) or GABA (vGAT + SYN, C). B, D, Graphs depicting the mean ± SEM density of synaptic (SYP-positive) contacts immunoreactive for vGluT2 (B) or vGaT (D). *P < 0.05. Scale bars = 5 µm.