Table 2.
Mean ± SEM percentage of kisspeptinergic, non-kisspeptinergic VGLUT2 cells and non-kisspeptinergic VGAT cells containing RNA transcripts in the ARC of PNV male (n = 5), PNV female (n = 4), and PNA female (n = 6) mice
| AMHR2 | AR | ESR1 | FOS | OPRK1 | PDYN | PGR | VGLUT2 | VGAT | TAC2 | TAC3R | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| KISS1 cells | PNV Male | 0.5 ± 0.2 | 88.5 ± 1.9 b | 92.5 ± 2.3 | 15.4 ± 5.1 | 64.4 ± 3.2 | 93.1 ± 2.1 | 97.4 ± 0.8 | 95.7 ± 1.4 | 48.0 ± 4.8 | 80.0 ± 1.8 b | 72.3 ± 5.0 |
| PNV Female | 0.5 ± 0.3 | 58.2 ± 4.4 | 95.8 ± 1.0 | 15.9 ± 3.2 | 58.5 ± 5.4 | 93.7 ± 2.1 | 97.2 ± 0.9 | 90.5 ± 4.5 | 53.4 ± 4.4 | 90.1 ± 1.7 | 81.5 ± 1.4 | |
| PNA Female | 0.8 ± 0.7 | 83.2 ± 2.3 b | 95.7 ± 1.2 | 15.0 ± 1.6 | 79.9 ± 2.7 a,b | 91.0 ± 1.4 | 91.7 ± 1.5 a,b | 88.2 ± 2.7 | 51.7 ± 4.7 | 91.3 ± 1.7 a | 86.0 ± 2.6 a | |
| VGLUT2 cells | PNV Male | 0.6 ± 0.2 | 59.5 ± 3.4 | 41.3 ± 5.4 | 17.5 ± 2.5 | 35.0 ± 5.1 | 49.6 ± 7.1 | 78.4 ± 3.1 | N/A | 44.5 ± 4.7 | 6.8 ± 1.4 | 7.9 ± 1.4 |
| PNV Female | 1.1 ± 0.3 | 59.8 ± 1.7 | 53.9 ± 5.4 | 19.0 ± 4.1 | 24.2 ± 3.3 | 51.7 ± 3.5 | 76.1 ± 3.8 | N/A | 43.3 ± 4.3 | 7.2 ± 0.9 | 7.6 ± 1.4 | |
| PNA Female | 0.5 ± 0.1 | 66.8 ± 2.6 | 44.2 ± 3.2 | 16.5 ± 1.6 | 44.4 ± 5.8 b | 48.4 ± 3.0 | 72.9 ± 3.4 | N/A | 41.8 ± 5.5 | 7.6 ± 1.0 | 12.8 ± 3.5 | |
| VGAT cells | PNV Male | 0.2 ± 0.1 | 46.8 ± 3.5 | 38.7 ± 6.0 | 18.9 ± 2.7 | 28.1 ± 5.1 | 30.7 ± 3.6 | 74.3 ± 3.1 | 26.7 ± 44 | N/A | 2.4 ± 0.1 | 3.1 ± 0.2 |
| PNV Female | 0.3 ± 0.03 | 44.3 ± 3.0 | 49.3 ± 3.1 | 18.1 ± 4.9 | 19.9 ± 1.5 | 26.7 ± 1.9 | 72.0 ± 4.2 | 18.0 ± 3.5 | N/A | 3.8 ± 0.6 | 4.6 ± 1.5 | |
| PNA Female | 0.3 ± 0.1 | 55.9 ± 2.7 | 48.8 ± 2.7 | 14.8 ± 1.2 | 36.3 ± 6.1 | 28.1 ± 1.2 | 71.9 ± 3.5 | 20.6 ± 2.6 | N/A | 4.0 ± 0.4 | 5.6 ± 1.1 |
Abbreviations: ARC, arcuate nucleus; N/A, not assessable; PNA, prenatal androgen-treated; PNV, prenatal vehicle; SEM, standard error of the mean.
a significantly different vs PNV male,
b significantly different vs PNV female.