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. Author manuscript; available in PMC: 2022 Jul 8.
Published in final edited form as: Cell. 2021 Jun 10;184(14):3612–3625.e17. doi: 10.1016/j.cell.2021.05.008

Figure 3. Integrity of the Tudor domain is required for formation of endogenous nuclear MLOs by full-length SMN.

Figure 3.

A) Images comparing HeLa cells transfected with non-targeting oligo, siSMN oligo pool, wild type (WT) rescue construct, and mutant rescue constructs. Grayscale bar given in analog-digital units. Scale bar= 10 μm. B) Quantification of SMN-containing MLOs per nucleus in each condition shown in (A). Kruskal-Wallace test for multiple comparisons, p = 2.19 (10−12) C) Quantification of SMN condensate radius given in pixels for each condition shown in (A). Kruskal-Wallace test for multiple comparisons, p = 9.33 (10−15). D) Integrated fluorescence intensity of labeled SMN in cells for each condition, units given as analog-digital units (ADU). Individual samples were compared with Dunn’s post hoc analysis and indicated as not significant (n. s.) where p > 0.05 and **** where p < 0.0001.