KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
|
| ||
| Antibodies | ||
|
| ||
| Anti mCherry | Invitrogen | #PA5-34974; RRID: AB_2552323 |
| Anti coilin (H-300) | Santa Cruz Biotechnology | #sc-32860; RRID: AB_2081431 |
| Anti-coilin | Abcam | #ab210785 |
| Anti sDMA (SYM10) | Millipore Sigma | #07-412; RRID: AB_11212396 |
| Anti sDMA (SYM11) | Millipore Sigma | #07-413; RRID: 310595 |
| Anti aDMA (ASYM24) | Millipore Sigma | #07-414; RRID: AB_310596 |
| Anti SMN (2B1) | Abcam | #ab5831; RRID: 305144 |
| Anti GFP | Invitrogen | #A11122; RRID: AB_221569 |
| Anti GAPDH | Santa Cruz Biotechnology | #sc-25778; RRID: AB_10167668 |
| Anti Sm (Y12) | Laboratory of Joan Steitz; Pettersson et al., 1984 | N/A |
| Anti 2,2,7-Trimethylguanosine (K121) | Calbiochem | #NA02; RRID: AB_213109 |
| Anti SNRPC | Abcam | #ab82862; RRID: AB_2193870 |
| Anti U1-70K (CB7) | Laboratory of Doug Black; Listerman et al., 2006 | N/A |
| Anti-rabbit IgG Horseradish Peroxidase-linked | GE HealthCrae | #NA934; RRID: AB_772206 |
| Alexa Fluor 488-conjugated Donkey Anti-Rabbit | Jackson ImmunoResearch | #711-545-152; RRID: AB_2313584 |
| Alexa Fluor 488-conjugated Donkey Anti-Mouse | Jackson ImmunoResearch | #715-545-150; RRID: AB_2340850 |
| Anti-Mouse IgG - Atto 594 | Sigma | #76085; RRID: AB_1137653 |
| Anti-Rabbit IgG - Atto 594 | Sigma | #77671; RRID: AB_1137663 |
| Anti-Mouse IgG - Atto 647N | Sigma | #50185; RRID: AB_1137661 |
| Anti-Rabbit IgG - Atto 647N | Sigma | #40839; RRID: AB_1137669 |
|
| ||
| Chemicals, peptides, and recombinant proteins | ||
|
| ||
| MS-023 | Sigma | SML1555 |
| EPZ015666 | Sigma | SML1421 |
|
| ||
| Deposited data | ||
|
| ||
| Optodroplet Live Imaging Dataset - mCherry Control |
This Study | 4DNucleome: 4DNESVT8QSLU |
| Optodroplet Live Imaging Dataset- SMNTud | This Study | 4DNucleome: 4DNES9JHBEZC |
| Optodroplet Live Imaging Dataset - hnRNPA1IDR | This Study | 4DNucleome: 4DNES49WH1QD |
| Optodroplet Live Imaging Dataset - SMNTud Mock | This Study | 4DNucleome: 4DNESPZNHYSY |
| Optodroplet Live Imaging Dataset- SMNTud Treated with MS023 and EPZ015666 |
This Study | 4DNucleome: 4DNESM8F8PP5 |
| Optodroplet Live Imaging Dataset - SMNTud W102L |
This Study | 4DNucleome: 4DNESGOUKUHY |
| Optodroplet Live Imaging Dataset- SMNTud Y109L | This Study | 4DNucleome: 4DNESRORWX8M |
| Optodroplet Live Imaging Dataset- SMNTud Y130D | This Study | 4DNucleome: 4DNESGSHWGUK |
| Optodroplet Live Imaging Dataset - SMNTud E134K | This Study | 4DNucleome: 4DNESQJS4P53 |
| Optodroplet Live Imaging Dataset - SMNTud F118L | This Study | 4DNucleome: 4DNESLBSY7UC |
| Optodroplet Live Imaging Dataset - Spf30Tud | This Study | 4DNucleome: 4DNESZZ8T7O9 |
|
| ||
| Experimental models: Cell lines | ||
|
| ||
| HeLa-Kyoto | Neugebauer Lab | RRID: CVCL_1922 |
| NIH 3T3 | ATCC | CRL-1658 |
| HEK293FT | ThermoFisher | R70007 |
| Coil/ mouse embryonic fibroblasts | Laboratory of Greg Matera; Tucker et al., 2001 | N/A |
|
| ||
| Oligonucleotides | ||
|
| ||
| siSMN #1 | Thermo | #4427037 s445227 |
| siSMN #2 | Thermo | #4427037 s531465 |
| siSMN #3 | Thermo | #4427037 s531467 |
|
| ||
| Recombinant DNA | ||
|
| ||
| mCh-Cry2 | Laboratory of Clifford Brangwynne | N/A |
| FUSIDR | Laboratory of Clifford Brangwynne | N/A |
| hnRNPA1IDR | Laboratory of Clifford Brangwynne | N/A |
| SMN-Cry2 | This study | N/A |
| SMNIDR-N | This study | N/A |
| SMNTud | This study | N/A |
| SMNIDR-C | This study | N/A |
| dmTudorTud | This study | N/A |
| Aub3 | This study | N/A |
| Aub3(KG) | This study | N/A |
| Spf30Tud | This study | N/A |
| Tdrd1Tud#2 | This study | N/A |
| Tdrd1Tud#3 | This study | N/A |
| Tdrd1Tud#4 | This study | N/A |
| Tdrd3Tud | This study | N/A |
| Tdrd4Tud#1 | This study | N/A |
| Tdrd4Tud#4 | This study | N/A |
| Tdrd6Tud#5 | This study | N/A |
| Tdrd6Tud#6 | This study | N/A |
| Tdrd8Tud | This study | N/A |
| Tdrd9Tud | This study | N/A |
| Snd1Tud | This study | N/A |
| Recoded SMN-Myc | This study | N/A |
| Recoded SMN-Myc (E134K) | This study | N/A |
| Recoded SMN-Myc (Y109L) | This study | N/A |
|
| ||
| Software and algorithms | ||
|
| ||
| Python Microscopy (PYME) | David Baddeley | https://Python-microscopy.org |
| FIJI | Schindelin et al., 2012 | https://Fiji.sc |
| Cell Profiler | Carpenter et al., 2006 | https://Cellprofiler.org |
| RaptorX | Kallberg et al., 2012 | https://Raptorx.uchicago.edu |
| Clustal Omega | Sievers et al., 2011 | https://Ebi.ac.uk |
| Imspector | Abberior Instruments | imspector.abberior-instruments.com |
| ConSurf | Berezin et al., 2004 | https://Consurf.tau.ac.il |
| Imaris | Oxford Instruments | https://Imaris.oxinst.com |