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. 2021 Aug 24;14:3393–3403. doi: 10.2147/IDR.S306878

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© 2021 Anis et al.

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PCR-RFLP based detection of mutations A2142G and A2143G in 1400 kb amplicon of 23S rRNA gene of H. pylori with BsaI and MboII restriction enzymes, respectively. Lane 1 shows the new MboII RFLP pattern, ie, approximately 1000bp and 400 bp fragments appears in the presence of A1761G mutation and sequencing also confirmed this mutation (see Figure 3); Lane 2 shows the clarithromycin resistant restriction profile with BsaI, ie, 700bp, 400bp and 300bp fragments indicate the presence of A2143G mutation; Lane 3 shows the clarithromycin resistant profile with MboII, ie, 710bp and 690 fragments indicate the presence of A2142G mutation, M.W, 100bp molecular weight marker.