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. 2021 Aug 24;17:2791–2802. doi: 10.2147/NDT.S292471

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© 2021 Guo et al.

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Sh-JAK2 suppressed neuronal cell death and ischemic brain injury. (A–E) The neuronal cells were transfected with sh-JAK2 or sh-NC, and cells were exposed to OGD/R or not. (A) JAK2 mRNA level was evaluated by qRT-PCR (n = 3). (B and C) The protein levels of JAK2 (B) and p-STAT3 (C) were evaluated by Western blot (n = 3). (D) Cell viability was detected by MTT assay (n = 3). (E) The apoptosis was detected by flow cytometry (n = 3). (F–I) Cortical injection of sh-JAK2 or sh-NC, and rats were then exposed to MCAO/R or not. (F) JAK2 mRNA level was evaluated by qRT-PCR (n = 6). (G) JAK2 protein level was evaluated by Western blot (n = 6). (H) Neurobehavioral outcomes (n = 6). (I) Infarct volumes (n = 6). Data were presented as mean ± SD. *p < 0.05, **p < 0.01.