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CNP downregulates pro-inflammatory factors by affecting the infiltration and proliferation of macrophages. (A) IHC to detect macrophages in caput epididymis. (B) Histogram showed the degree of macrophage infiltration. (C) CCK-8 assay of the proliferative activity of inflammatory macrophages. (D) mRNA levels and concentrations of IL-6, TNF-α, and IL-1β. The data shown are representative of multiple experiments (mean ± SD, n = 3, *P < 0.05, **P < 0.01).
