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. 2021 Aug 12;11:711842. doi: 10.3389/fcimb.2021.711842

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Copyright © 2021 Mei, Kang, Zhang, He, Liao and Huang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CNP represses NF-kB to release pro-inflammatory factors by the cGMP/PKG signaling pathway in macrophages. (A) Immunofluorescence showed the location of NPR-B in RAW264.7macrophages. (B) ELISA revealed intracellular cGMP level in macrophages cultured with CNP for 6 h. (C) Western blot analysis showed NF-kB protein expression after incubation with CNP, 8-Br-cGMP or KT5823 alone or in combination and was quantified in images. (D) qPCR showed the mRNA levels of the downstream pro-inflammatory cytokines IL-6, TNF-α, and IL-1β. The data shown are representative of multiple experiments (mean ± SD, n = 3, *P < 0.05, **P < 0.01, ***P < 0.001).