Figure 2:

Expression of coat proteins, ORF13 and ORF14, in pET15b and pET28a vectors. A) Plasmids pET15b-ORF14 or pET28a-ORF13 and B) plasmids pET15b-ORF13 or pET28a-ORF14 were used to transformed C41 E. coli cells. The bacteria were grown at 37 °C and protein express was induced with 0.5 mM IPTG. Cell pellets were lysed with 8 M Urea and loaded on SDS-PAGE gel for analysis. Gels were stained with Coomassie blue. Arrows indicate coat protein bands of ORF13 (18.81 KD) and ORF14 (32.01 KD). (−) is uninduced culture and (+) is induced IPTG culture. M=Molecular weight marker.