FIG. 2.

High n-3 PUFA diet suppresses Iba-1 expression after rCHIMERA. (A) Representative photomicrographs of activated microglia indicated by Iba-1 expression in optic tract (OT), corpus callosum (CC), and hippocampus (HP) obtained from high (Ade) or low (Def) n-3 PUFA diet groups at 2 months after injury by rCHIMERA (rCHI). Regions of interest are outlined with dashed lines. (B) Quantitative analysis of Iba-1 expression. Data are normalized to Ade-Sham values and expressed as mean ± standard error (n = 4; ***p < 0.001 vs. Ade-Sham). Iba-1, ionized calcium-binding adaptor molecule 1; PUFA, polyunsaturated fatty acid; rCHIMERA, repeated CHIMERA.