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. 2021 Aug 17;33(4):302–305. doi: 10.1089/acu.2020.1513

Table 1.

Lower Reference Limits of Semen Characteristics3

Parameters Lower reference limits
Semen volume (mL) 1.5 (1.4–1.7)
Total sperm number (106/ejaculate) 39 (33–46)
Sperm concentration (106/mL) 15 (12–16)
Total motility (PR + NP, %) 40 (38–42)
Progressive motility (PR, %) 32 (31–34)
Vitality (live spermatozoa, %) 58 (55–63)
Sperm morphology (normal forms, %) 4 (3.0–4.0)
Other consensus threshold values
 pH ≥ 7.2
 Peroxidase-positive leukocytes (106/mL) < 1
 MAR test (motile spermatozoa with bound particles, %) < 50
 Immunobead test (motile spermatozoa with bound beads, %) < 50
 Seminal zinc (μmol/ejaculate) ≥ 2.4
 Seminal fructose (μmol/ejaculate) ≥ 13
 Seminal neutral glucosidase (mU/ejaculate) ≥ 20

PR, progressive; NP, nonprogressive; MAR, mixed antiglobulin reaction.