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. 2021 Jun 23;10(13):e020502. doi: 10.1161/JAHA.120.020502

Figure 6. Immunosuppressive characteristics of ITE‐primed tDCs.

Figure 6

A, Splenocytes were cocultured with tDCs or mDCs and stimulated with or without ITE for 72 hours at 37°C. B, The bar graphs show the mean percentage of CD4+CD25+Foxp3+ cells. C, Relative Foxp3 mRNA expression was measured by quantitative real‐time polymerase chain reaction. Foxp3 mRNA expression was quantified relative to that of GAPDH. Data are presented as mean±SEM and were compared using 1‐way ANOVA (n=5 per group). APC indicates allophycocyanin; Foxp3 indicates forkhead box protein P3; ITE, 2‐(1’H‐indole‐3’‐carbonyl)‐thiazole‐4‐carboxylic acid methyl ester; mDCs, mature dendritic cells; ns, not significant; and tDCs, tolerogenic dendritic cells. *P<0.05. **P<0.01. ***P<0.001.