a, Sublineage diagram showing the fate of ABplpappap (*)
in wild-type, ced-3(lf) and
ced-3(lf)+extrusion-defective animals. b,
c, Merged epifluorescence and Nomarski micrographs of the
pharyngeal region of (b)
nIs433[Ppgp-12::4xNLS-GFP];
ced-3(lf) and (c) extrusion-defective nIs433;
ced-3(lf); pig-1(RNAi) animals (ref. 3). d, The
percentages of animals with the Tex phenotype in ced-3(lf)
animals after the indicated RNAi treatment. ‡, identified from screen
shown in Extended Data Figure 1a; others
identified from candidate RNAi screens. e, Merged epifluorescence
and Nomarski micrographs of the pharyngeal region of nIs433;
ced-3(lf) animals after the indicated RNAi treatment.
f, g, Ventral and virtual lateral views of
ced-3(lf); stIs10026[his-72::GFP];
nIs632[Pegl-1::mCherry::PH]
embryos after the indicated RNAi treatment at the indicated time point.
tve – time point of ventral enclosure. h,
Percentage of animals of the indicated genotype displaying the Tex phenotype.
i, Cell-lineage diagram showing number of animals with the Tex
phenotype carrying the cye-1(+)-rescuing array
nEx3043 in the indicated lineage or cell in 10
cye-1(lf); ced-3(lf);
nIs434[Ppgp-12::4xNLS-GFP]
animals. j, k, Two confocal fluorescence micrographs
of one of 10 cye-1(lf); ced-3(lf); nIs434; nEx3043 genetic
mosaic worms represented in (i). Arrowheads, excretory or ectopic
excretory-like cells in (b, c, e); ABplpappap in
(f, g). A, anterior; R, right; D, dorsal; V,
ventral. Scale bars, 10 μm.