a, c, d, Representative
micrographs of (a) α-γH2AX, (c)
α-pATR and (d) α-p53 immunofluorescence signal in
(c) vehicle- or HU-treated MDCK-II cells, and
(a, d) vehicle-, HU- or Nutlin3-treated
MDCK-II cells. DNA is stained with Hoechst. Scale bars, 20 μm.
b, Quantification of extrusions per h after the indicated
treatment. n = 10, 6, 5, 5 and 5 (biological replicates) each for control,
PFT, zVAD-FMK, SB 218078 and PF477736 treatments, respectively. Each data
point represents a separate experiment. These data were collected and
analyzed for statistical significance with the data in Fig. 4g. P values are indicated;
n.s., not significant. e, Quantification of α-p53
immunofluorescence signal in vehicle-control-, HU-, or Nutlin-3-treated
MDCK-II cells. n = 9, 7 and 5 (biological replicates) for vehicle, HU and
Nutlin3, respectively. Each data point represents mean fluorescence
intensity signal from one image of 100s of cells. Kruskal-Wallis one-way
ANOVA followed by Dunn’s correction was performed. P
values are indicated. Data in (b, e) are
represented as mean ± S.D.