a, Relative nuclear/cytoplasmic localization of a tDHB-GFP
fusion protein in the indicated cell-cycle phases10. b, localization pattern of
GFP::PCN-1 in a typical C. elegans embryonic cell in the
indicated cell-cycle phase. c, Nuclear:cytoplasmic ratio of
tDHB-GFP fluorescence intensity in ABplpappap in
heSi192[Peft-3::tDHB-GFP];
ced-3(lf);
nIs861[Pegl-1::mCherry::PH]
embryos after the indicated RNAi treatment. d, Quantification of
the coefficient of variation of GFP::PCN-1 fluorescence intensity in
ced-3(lf);
isIs17[Ppie-1::GFP::pcn-1];
nIs861 embryos after indicated RNAi treatment. e,
Time-lapse confocal fluorescence micrographs of GFP::PCN-1 fluorescence in
ABplpappap (arrowhead) in ced-3(lf); isIs17; nIs861 embryos at
the indicated times after treatment with control RNAi. Arrow, unidentified
extruding cell. f, Micrographs of virtual lateral section of
ced-3(lf); nIs861; stIs10026[his-72::GFP] embryos showing
either ABplpappap (arrowhead) or its daughters (arrowheads) after indicated RNAi
treatment. g, Quantification of the ratio of maximum area occupied
by ABplpappap to that occupied by its sister, ABplpappaa, in ced-3(lf);
ltIs44[Ppie-1::mCherry::PH];
stIs10026 embryos after the indicated RNAi treatment. Right inset,
magnified view of ABplpappap or its daughters. Left inset in (e),
magnified view of unidentified extruding cell. Scale bars, 10 μm in all
micrographs except (b) and 2 μm in (b). A,
anterior; R, right; V, ventral. n=10 embryos (biological replicates) for each
RNAi treatment in (c, d, g). Data in
(c, d, g) are represented as mean
± S.D. Ordinary one-way ANOVA with Dunnett’s correction for
multiple comparisons (c, d, g).
P values are indicated; n.s., not significant.