Figure 3.

Comparison of N/TERT-1 organotypic skin models and normal human skin

HE stain of (A) Organotypic culture with N/TERT-1 cells, and (B) of normal human skin. Immunohistochemical staining for the proliferation marker Ki67 of (C) N/TERT-1 organotypic culture, and (D) normal human skin. Immunofluorescence staining of (E) N/TERT-1 WT organotypic tissue and (F) normal human skin for differentiation marker keratin 10 in green and nuclei stained with DAPI in blue. (G) Diagram illustrating equal number of basal Ki67 positive cells in human skin sections and sections of N/TERT-1 organotypic cultures. Data are presented as mean ± SEM. No significant difference was observed between human skin and N/TERT-1 tissues using unpaired T-test. Technical replicates = 3-4, biological replicates = 3. Scale bar represents 50 μm.