REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
One Shot™ Stbl3™ Chemically Competent E. coli	Thermo Fisher	Cat#C7373-03
Chemicals, peptides, and recombinant proteins
Opti-MEM	Thermo Fisher (GIBCO)	Cat#31985062
Polybrene	Sigma Aldrich	Cat#TR-1003-G
Puromycin	Thermo Fisher (GIBCO)	Cat#A1113803
Blasticidin S HCl	Thermo Fisher (GIBCO)	Cat#A1113903
Pen Strep	Thermo Fisher (GIBCO)	Cat#15140122
DMEM	Thermo Fisher (GIBCO)	Cat#41966029
Keratinocyte-SFM	Thermo Fisher (GIBCO)	Cat#17005034
Dimethyl Sulfoxide	Sigma Aldrich	Cat#D2650
TrypLE	Thermo Fisher (GIBCO)	Cat#12605028
Bovine Pituitary Extract	Thermo Fisher (GIBCO)	Cat#13028014
Fetal Bovine Serum	HyClone	Cat#SH30071.03HI
EGF	Thermo Fisher (GIBCO)	Cat#PHG0314
Insulin	Sigma Aldrich	Cat#I6634
Hydrocortisone	Sigma Aldrich	Cat# H0888
Triidothryonine “T3”	Sigma Aldrich	Cat# T2752
Rat tail collagen I	Made in lab	N/A
L-Glutamine	Thermo Fisher (GIBCO)	Cat#A2916801
Hams F12	Thermo Fisher (GIBCO)	Cat#11765-054
Adenine	Sigma Aldrich	Cat#A8626
Cholera Enterotoxin	Sigma Aldrich	Cat#C8180
10X MEM	Thermo Fisher (GIBCO)	Cat#11430-030
Sodium Bicarbonate	Merck	Cat#6392
Gentamycine (50mg/mL)	GIBCO	Cat#15750037
Methyl (3-(R)-Fluoro-5-N-ethyloxycarbonyl- 2,4,7,8,9-penta-O-acetyl-3,5-dideoxy-D-a/ b-glycero-D-galacto-2-nonulopyranose) onate(Ac5SiaFEtoc)	Thomas J. Boltje, Cluster for Molecular Chemistry, Institute for Molecules and Materials, Radboud University Nijmegen, the Netherlands	N/A
BsmBI	NEB	Cat#R0580
T4 DNA ligase Buffer (10x)	Thermo Fisher	Cat#B69
Critical commercial assays
NucleoBond Xtra Midi EF	Macherey-Nagel	Cat#740420.50
CoboXtract	Cobo Technologies	Cat#C20101
Experimental models: Cell lines
HEK293T	ATCC	Cat# CRL-3216, RRID:CVCL_0063
MRC-5	ATCC	Cat# CCL-171, RRID:CVCL_0440
N/TERT-1	James G. Rheinwald, Harvard Institute of Medicine	N/A
Recombinant DNA
pCMV-VSV-G	Addgene	RRID:Addgene_845 4
lentiCRISPR v2	Addgene	RRID:Addgene_529 61
psPAX2	Addgene	RRID:Addgene_122 60
Software and algorithms
Peak Scanner Software V1.0	Thermo Fischer	Cat#: 4381867
Other
Syringe filter 0.45-μm	Sartorius	Cat#17598-K
Deep well plates	Corning	Cat#355467
Cell culture insert, PET membrane, 3.0 μm pore size	Corning	Cat#353092
Airlift pads (cut in circles and autoclaved)	Whatman, GE Healthcare	Cat# 10382461