Reagent | Final concentration | Amount |
---|---|---|
Keratinocyte-SFM | n/a | 500 mL |
CaCl2 (1000×) | 0.3 mM | 500 μL |
BPE (16.8 mg/mL) | 25 μg/mL | 750 μL |
EGF (10 μg/mL) | 0.2 ng/mL | 10 μL |
Total | n/a | 500.91 mL |
Store at 4°C for up to 3 months
Reagent | Final concentration | Amount |
---|---|---|
Keratinocyte-SFM | n/a | 500 mL |
CaCl2 (1000×) | 0.3 mM | 500 μL |
BPE (16.8 mg/mL) | 25 μg/mL | 750 μL |
EGF (10 μg/mL) | 0.2 ng/mL | 10 μL |
Total | n/a | 500.91 mL |
Store at 4°C for up to 3 months