| Reagent | Amount |
|---|---|
| Opti-MEM (100 μM) | 200 μL |
| PEI (1 mg/mL) | 8 μL |
| pCMV-VSV-G plasmid | 0.6 μg |
| psPAX2 plasmid | 0.6 μg |
| gRNA in lentiCRISPR-v2 plasmid | 0.8 μg |
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