Fig. 2.

Protein expression levels of tPSA and COL1A1 in human PCa specimens. PSA and COL1A1 levels were quantified by western blot analysis in surgical resections from RP. Specimens are from our institutional biobank. Equal micrograms of tissue lysate were loaded. (A) Representative blot images showing COL1A1 expression from samples with different PSA levels. COL1A1 and PSA were quantified on the same blot. (B) Plots showing quantification of COL1A1 and cleavage products of COL1A1 according to PSA levels. (C) Table showing number of cases, pathological features and statistical differences among groups with low or high PSA expression. (D) Plots showing protein PSA levels, normalized to the level of actin, according to grade, stage and lymph node positivity (LN1). Mean values ± SEM are reported. *p < 0.05; **p < 0.01; ****p < 0.0001 Mann-Whitney test.