Figure 2.

TGF-β increases cytosolic calcium concentrations in A549 cells. (A–D) A549 cells were treated with 10 ng/ml human recombinant TGF-β for 24 hours. Cells were subjected to Fluo-3 AM staining and flow cytometry measurement. Representative data (A) and quantification analysis of the positive percentage of fluorescence signals (B) or geometric mean (C) were shown. (D) The experiment was biologically replicated and fold change of the replicate experiment was calculated. Student’s t-test was performed for analyzing statistical significance. ^**P < 0.01.