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. 2021 Jun 17;10(4):733–741. doi: 10.1093/toxres/tfab055

Figure 3.

Figure 3

TGF-β induces NFATc1 and NFATc2 activation via promotion of the calcium–calcineurin axis. (A) A549 cells were treated with 10 ng/ml TGF-β for 24 hours. The cells were then subjected to QPCR analysis for NFATc1, NFATc2, or NFATc3. GAPDH was utilized as the housekeeping gene. (B–D) A549 cells were treated with 10 ng/ml TGF-β, together with 50 μM BAPTA or 1 μM CsA as indicated. The cells were then subjected to QPCR analysis for NFATc1 (B), NFATc2 (C), or NFATc3 (D). GAPDH was utilized as housekeeping gene. The statistical significance in all of the panels was analyzed by Two-way ANOVA. N.S., not significant; *P < 0.05, **P < 0.01, ***P < 0.001.