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. 2021 Jun 17;10(4):733–741. doi: 10.1093/toxres/tfab055

Figure 4.

Figure 4

PQ but not DQ drives EMT and is largely reversed by blockage of the calcium–calcineurin axis in A549 cells. (A–B) A549 cells were treated with 800 μM PQ, together with 50 μM BAPTA or 1 μM CsA as indicated, for 24 hours. The cells were then subjected to QPCR analysis for E-Cadherin (A) and Vimentin (B). (C–G) A549 cells were treated with 800 μM DQ for 24 hours. The cells were then subjected to QPCR analysis for E-Cadherin (C), Vimentin (D), NFATc1 (E), NFATc2 (F), and NFATc3 (G). GAPDH was utilized as the housekeeping gene. The statistical significance was analyzed by Two-way ANOVA. N.S., not significant; *P < 0.05, **P < 0.01, ***P < 0.001. (H) Retrospectively analysis of the blood calcium levels in patients suffering PQ or DQ poisoning. Student’s t-test was performed for analyzing statistical significance. *P < 0.05.