| Reagent | Final concentration | Volume |
|---|---|---|
| H20 | n/a | to 20 uL |
| linearized plasmid | 1 ug | to 1 ug |
| 10× Transcription Buffer | 1 | 2 uL |
| 10 mM ATP | 1 uL | |
| 10 mM CTP | 1 uL | |
| 10 mM GTP | 1 uL | |
| 10 mM UTP | 0.6 uL | |
| bio-11-UTP | 0.4 uL | |
| T7 enzyme | 1× | 2 uL |
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