Caspase-3 assay in 4T1 breast cancer stem cells when co-cultured with CD4+ and CD8+ T cells or just CD8+ cells
The first row shows the 4T1 cells cultured with CD8+ T cells only with one of the following, in order from left to right: PBS, non-transfected DCs, or transfected DCs. The second row shows the 4T1 cells cultured with both CD4+ and CD8+ T cells with one of the following, in order from left to right: PBS, non-transfected DCs, or transfected DCs. 4T1 cells co-cultured with CD4+ and CD8+ T cells show significantly more caspase-3 than when the 4T1 cells were co-cultured with CD8+ T cells only across all three groups.