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. Author manuscript; available in PMC: 2021 Aug 30.
Published in final edited form as: J Physiol. 2020 May 30;598(16):3395–3415. doi: 10.1113/JP279009

Figure 6. Immunofluorescence showing that the expression of NBCn1 and NBCn2 in the mTAL of rat kidney is increased by NaHCO3.

Figure 6.

The rats were treated with NaHCO3 for 7 days. Cryo-sections of the kidney were double-stained with anti-NBCn1 (or anti-NBCn2) and anti-α1. A1−A4, expression of NBCn1 in the outer medulla (OM) of the control rat. B1−B4, expression of NBCn1 in the OM of NaHCO3-treated rat. C1−C4, expression of NBCn2 in the OM of control rat. D1−D4, expression of NBCn2 in the OM of NaHCO3-treated rat. The red shows the expression of NBCn1 or NBCn2. The green shows the expression of α1 of the Na+-K+ pump. The blue in the merge represents the staining of the nuclei by DAPI. The data are representative of three independent experiments.